About the Analytical Chemistry Laboratory

The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers.

The Analytical Chemistry Laboratory performs custom testing and has a standard fee schedule for services. The fee structure is based on the amount of time required to process the samples (extraction, derivatization, etc.) and the cost of supplies and reagents. For non-NIH sponsored projects (MSM and outside institutions and corporations), labor costs are assessed. A complete listing of currently available tests or cost per requested assay can be emailed or faxed upon request. Additionally, since the Analytical Chemistry Laboratory performs custom testing, if the requested assay is not on the list, please contact the core leader directly for test availability and the most up-to-date pricing.

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About Lab Services Research Resources

The Biomedical Technology Service Laboratory (BTSL) is divided into seven labs housing multiuse equipment and a monoclonal/polyclonal antibody production lab. Housed within seven laboratories, located throughout the Westview campus, are small and large multiuse equipment such as plate readers, ultracentrifuges, spectrophotometers.

Group and one-on-one instruction in the use of this instrumentation is offered by core personnel. Also offered within these labs are general reagents such as dry ice and liquid nitrogen, ultra freezer space, and support for technique/protocol development. Also housed within the BTSL is amonoclonal and polyclonal antibody production lab. Examples of antibodies made by this lab include but are not limited to: antibodies against conjugated synthetic peptides, chemokines, cytokines, cellular antigens, viral proteins, protein bands excised from PAGE gels.

Flow Cytometry, FACS, and magnetic-bead cell-sorting lab houses a BD FACS Aria II and Miltenyi Biotec autoMACS Pro Seperator. Certfied BD- and Miltenyi-trained staff are available for consultation, acquiring data, or sorting cells.

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Analytical Services

1. Custom sample analysis by High-performance liquid chromatography (HPLC).
2. ELISA
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology

Equipment/Technology

1. High Performance Liquid Chromatography (HPLC): Custom analyses by HPLC can include (but not limited to): amino acids; thiols (total, oxidized, reduced); chiral and racemic compounds such as warfarin and clenbuterol; etc.

  • Agilent 1100 series HPLC equipped with a diode array detector (DAD), variable wavelength detector (VWD), 1046 series fluorescence detector.
  • Agilent 1100 series HPLC system equipped with 1100 series fluorescence detector.

    • 2. Molecular Devices SpectroMax Plus microtiter plate reader and variable wavelength UV-Visible spectrophotometer to perform Colorimetric and Enzyme-linked Immunosorbent assays.
    3. NOVA Electrolytes Analyzer for analyses of Na+, P+, TCa+, Li+, Cl- in biological fluids.

    Analytical Chemistry Laboratory Team & Lab Services Research Resources Team

    Winston E. Thompson, Ph.D., M.S.

    Co-Investigator and Southeast Training Hub Co-Chair, Director Of Research, Obstetrics & Gynecology And Director, Mentoring Academy

    Michael D. Powell

    Proteomics Core Leader and Professor of Microbiology, Biochemistry & Immunology

    Kameswara Badri, Ph.D.

    Assistant Professor, Pharmacology & Toxicology (CVRI)